Epigenetic Methylation Station

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Total Cellular RNA Extraction Protocol

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METHODS and MATERIALS: A Protocol for Total Cellular RNA Extraction

Total cellular RNA is isolated from 60-80% confluent cell monolayers using the RNeasy Midi Kit (QIAGEN Inc., Valencia , CA ) and following the manufacturer’s instructions. Total RNA concentration and its purity can and should be determined spectrophotometrically.

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