Epigenetic Methylation Station

Your source for information, protocols, and bioinformatics about epigenetics and DNA methylation.

Epigenetic Methylation Station

Your source for information, protocols, and bioinformatics about epigenetics and DNA methylation.
Restriction Endonuclease Enzymes 10Sep

Restriction Endonuclease Enzymes

Restriction Endonuclease Enzymes to Study DNA Methylation and Epigenetic Changes A few restriction enzymes are known to differential cleave methylated versus non-methylated DNA. These enzymes can be used to help you. When selecting these restriction

Chromatin Immunoprecipitation 07Sep

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation Assay (ChIP) Chromatin Immunoprecipitation Assay, also known as the ChIP assay allows one to determine the mechanism of trascriptional repression, by immunoprecipitating chromatin with antibodies. ChIP involves formaldehyde treatment of a cell population

High Performance Liquid Chromatography 21Aug

High Performance Liquid Chromatography

High-performance Liquid Chromatography (HPLC) is used as a method to separate DNA or protein molecules more commonly, by weight as well as by conformation. This is done through differences of the molecules in their distribution

High Performance Capillary Electrophoresis 18Aug

High Performance Capillary Electrophoresis

This method allows for the accurate quantification of the total amount of 5-methylcytosines in the entire genome. High-performance Capillary Electrophoresis (HPCE) allows for separation of molecules, similar to the HPLC, but uses narrow-bore fused-silica capillary

Bisulphite Treatment Pyrosequencing 17Aug

Bisulphite Treatment Pyrosequencing

Bisulphite Treatment in Combination with Pyrosequencing This method is great for detecting minimal amounts of aberrant DNA methylation. Conventional SNP typing is used through the C to T typing, as light is emitted through an

LIST OF ABBREVIATIONS FOR EPIGENETICS 15Aug

LIST OF ABBREVIATIONS FOR EPIGENETICS

Ab Antibody 5-aza-dC 5-aza-2′-deoxycytidine Bp Base pair BSA Bovine serum albumin CpG Cytosine-phosphate-Guanine o C Degree Celsius CDNA Complimentary deoxyribonucleic acid DNA Deoxyribonucleic acid DNMT DNA methyltransferase DNTPs Deoxynucleoside triphosphates EDTA Ethylenediaminetetraacetic acid ELISA Enzyme-linked