Epigenetic Methylation Station

Your source for information, protocols, and bioinformatics about epigenetics and DNA methylation.

Epigenetic Methylation Station

Your source for information, protocols, and bioinformatics about epigenetics and DNA methylation.
Cell Lines and Cell Culture 24Sep

Cell Lines and Cell Culture

METHODS and MATERIALS: A Protocol for Cell Culture Cell lines can be easily purchased from the American Type Culture Collection. Cells are cultured in RPMI media (Gibco BRL, Gaithersburg , MD ) supplemented with glutamine

Stimulation Protocol 23Sep

Stimulation Protocol

Cell Stimulation Protocol Initially spin down cells, lyse for RNA extraction, RT-PCR for 20, 25, 30 cycles and do serial dilution of the RNA to avoid saturation (dilue: 50x, 100x, 25x, 500x). 1. For one

Total Cellular RNA Extraction Protocol 21Sep

Total Cellular RNA Extraction Protocol

METHODS and MATERIALS: A Protocol for Total Cellular RNA Extraction Total cellular RNA is isolated from 60-80% confluent cell monolayers using the RNeasy Midi Kit (QIAGEN Inc., Valencia , CA ) and following the manufacturer’s

Bisulphite Treatment Methylight 19Sep

Bisulphite Treatment Methylight

This method is great for detecting minimal amounts of aberrant DNA methylation. Primers are designed that overlap with CpG dinucleotides, and the DNA is treated with sodium bisulphite as mentioned previsouly. The advantage of this