Epigenetic Methylation Station

Your source for information, protocols, and bioinformatics about epigenetics and DNA methylation.

Epigenetic Methylation Station

Your source for information, protocols, and bioinformatics about epigenetics and DNA methylation.
PCR Fluorescence Melting Curve Analysis 30Sep

PCR Fluorescence Melting Curve Analysis

This method utilizes both treatment of DNA with sodium bisulfite initially, and melting curve analysis by using a thermal cycler, like the LightCycler from Roche Diagnostics, which uses a DNA-binding dye that binds double-stranded DNA,

PCR Methods to Study Epigenetic Changes 27Sep

PCR Methods to Study Epigenetic Changes

Epigenetic and Methylation Analysis of DNA by Polymerase Chain Reaction PCR Methods Southern Blotting Method Digestion with Methylation-Sensitive Enzymes followed by PCR Combined Bisulfite Restriction Analysis (COBRA) Bisulfite-PCR-SSCP (BiPS) Identification of CpG Islands Exhibiting Altered

Methylation Sensitive Restriction Enzymes Protocol 26Sep

Methylation Sensitive Restriction Enzymes Protocol

Methylation Sensitive Restriction Enzymes A Protocol for Digestion with Methylation-Sensitive Enzymes followed by PCR. This method uses methylation-sensitive restriction enzymes, to cleave DNA at specific methylated-cytosine residues which have lost their methyl group, that are

PCR Methods to Study Epigenetic Changes 25Sep

PCR Methods to Study Epigenetic Changes

Epigenetic and Methylation Analysis of DNA by Polymerase Chain Reaction PCR Methods Southern Blotting Method Digestion with Methylation-Sensitive Enzymes followed by PCR Combined Bisulfite Restriction Analysis (COBRA) Bisulfite-PCR-SSCP (BiPS) Identification of CpG Islands Exhibiting Altered