Epigenetic Methylation Station

Your source for information, protocols, and bioinformatics about epigenetics and DNA methylation.

Restriction Endonuclease Enzymes

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Restriction Endonuclease Enzymes to Study DNA Methylation and Epigenetic Changes

A few restriction enzymes are known to differential cleave methylated versus non-methylated DNA. These enzymes can be used to help you. When selecting these restriction enzymes, it is crucial to that they leave staggered ends, have a cut site that is different from the recognition site, and cannot be affected through potentially methylated target sequences.

C. Laird et al. Hairpin-bisulfite PCR: Assessing epigenetic methylation patterns on complementary strands of individual DNA molecules. Proc Nat Acad Sci (2004) 101: 204-209.

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