Epigenetic Methylation Station

Your source for information, protocols, and bioinformatics about epigenetics and DNA methylation.

High Performance Liquid Chromatography

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High-performance Liquid Chromatography (HPLC) is used as a method to separate DNA or protein molecules more commonly, by weight as well as by conformation. This is done through differences of the molecules in their distribution between a stationary phase and a mobile phase, which is carried out by the High-performance Liquid Chromatography (HPLC) Machine. Pressure changes in the system, allow for a greater resolution capacity. This method allows for the accurate quantification of the total amount of 5-methylcytosines in the entire genome.

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