Epigenetic Methylation Station

Your source for information, protocols, and bioinformatics about epigenetics and DNA methylation.

Bisulphite Treatment Methylight

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This method is great for detecting minimal amounts of aberrant DNA methylation. Primers are designed that overlap with CpG dinucleotides, and the DNA is treated with sodium bisulphite as mentioned previsouly. The advantage of this method, is that there is no need for any steps after PCR, as it uses fluoresence-based real-time PCR, Taqman, technology.

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