Epigenetic Methylation Station

Your source for information, protocols, and bioinformatics about epigenetics and DNA methylation.

Month: September 2018

Total Cellular RNA Extraction Protocol 21Sep

Total Cellular RNA Extraction Protocol

METHODS and MATERIALS: A Protocol for Total Cellular RNA Extraction Total cellular RNA is isolated from 60-80% confluent cell monolayers using the RNeasy Midi Kit (QIAGEN Inc., Valencia , CA ) and following the manufacturer’s

Bisulphite Treatment Methylight 19Sep

Bisulphite Treatment Methylight

This method is great for detecting minimal amounts of aberrant DNA methylation. Primers are designed that overlap with CpG dinucleotides, and the DNA is treated with sodium bisulphite as mentioned previsouly. The advantage of this

Restriction Endonuclease Enzymes 10Sep

Restriction Endonuclease Enzymes

Restriction Endonuclease Enzymes to Study DNA Methylation and Epigenetic Changes A few restriction enzymes are known to differential cleave methylated versus non-methylated DNA. These enzymes can be used to help you. When selecting these restriction

Chromatin Immunoprecipitation 07Sep

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation Assay (ChIP) Chromatin Immunoprecipitation Assay, also known as the ChIP assay allows one to determine the mechanism of trascriptional repression, by immunoprecipitating chromatin with antibodies. ChIP involves formaldehyde treatment of a cell population