Epigenetic Methylation Station

Your source for information, protocols, and bioinformatics about epigenetics and DNA methylation.

Month: August 2018

High Performance Liquid Chromatography 21Aug

High Performance Liquid Chromatography

High-performance Liquid Chromatography (HPLC) is used as a method to separate DNA or protein molecules more commonly, by weight as well as by conformation. This is done through differences of the molecules in their distribution

High Performance Capillary Electrophoresis 18Aug

High Performance Capillary Electrophoresis

This method allows for the accurate quantification of the total amount of 5-methylcytosines in the entire genome. High-performance Capillary Electrophoresis (HPCE) allows for separation of molecules, similar to the HPLC, but uses narrow-bore fused-silica capillary

Bisulphite Treatment Pyrosequencing 17Aug

Bisulphite Treatment Pyrosequencing

Bisulphite Treatment in Combination with Pyrosequencing This method is great for detecting minimal amounts of aberrant DNA methylation. Conventional SNP typing is used through the C to T typing, as light is emitted through an

LIST OF ABBREVIATIONS FOR EPIGENETICS 15Aug

LIST OF ABBREVIATIONS FOR EPIGENETICS

Ab Antibody 5-aza-dC 5-aza-2′-deoxycytidine Bp Base pair BSA Bovine serum albumin CpG Cytosine-phosphate-Guanine o C Degree Celsius CDNA Complimentary deoxyribonucleic acid DNA Deoxyribonucleic acid DNMT DNA methyltransferase DNTPs Deoxynucleoside triphosphates EDTA Ethylenediaminetetraacetic acid ELISA Enzyme-linked

Chromosomes 10Aug

Chromosomes

Packaging DNA in Higher Structural Forms Packing of DNA is required are DNA molecules in eukaryotes is very large. DNA therefore packs itself up into structures referred to as chromosomes. This allows DNA to be

Repetitive Sequences 09Aug

Repetitive Sequences

Repetitive Sequences and Epigenetics In comparison to CpG islands, which are found in the regulatory regions of genes, and are prime importance in the transcription of the regulators of cell growth and death, repetitive sequences,

Histone Code 08Aug

Histone Code

Histones are key regulators of gene expression, through numerous well-known chemical modifications. These include acetylation, phosphorylation, methylation, and ubiquitylation. For example, methylating and/or acetylating specific lysine residues of the nucleosomal cores of histones, such as

Insulators Loop Domain Model 07Aug

Insulators Loop Domain Model

Insulators and the Loop Domain Model In the loop domain model, inactive DNA is heterchromatin, with methylated DNA and hypoacetylated histones. Active DNA, is euchromatin, and has hyperacetylated histones and unmethylated DNA. Chromatin fibers are