TECHNIQUES TO STUDY EPIGENETICS

Links to Protocols, Methods and Techniques in order to study Epigenetic changes:

Epigenetic Cell Growth and Nucleic Acid Protocols:

Cell Lines and Cell Culture

Seeding, Passaging and Freezing Cells

Cell Stimulation Protocol

Preparation of Cytosolic Extracts

Total Cellular RNA Extraction

 

Polymerase Chain Reactions in Epigenetics:

Reverse Transcriptase Polymerase Chain Reaction RT-PCR

Methylation Specific PCR

Methods and Protocols to Analyze DNA Methylation and Epigenetic Changes:

Treatment of Cells with 5-Aza-2'-Deoxycytidine

Sodium Bisulfite Treatment of DNA

PCR Methods to study Epigenetics

Methylation Specific PCR

Methylation Sensitive Restriction Endonuclease Enzymes to study DNA Methylation and Epigenetic Changes

Methylation Sensitive Restriction Enzymes

Southern Blotting Method for DNA Methylation Detection

Combined Bisulfite Restriction Analysis COBRA

Bisulfite PCR Single Strand Conformation Polymorphism SSCP

PCR Fluorescence Melting Curve Analysis

Methylation Sensitive Single Nucleotide Primer Extension Ms-SNuPE

Identification of CpG Islands Exhibiting Altered Methylation Patterns (ICEAMP)

Hairpin-Bisulfite Polymerase Chain Reaction PCR

 

Histone Modification Protocols:

Chromatin Immunoprecipitation

 

Genome-Wide Approaches to studying Epigenetics:

Epigenomic Approaches