Methylation Sensitive Single Nucleotide Primer Extension Ms-SNuPE
This method allows you to analyze several CpG dinucleotides in a single reaction. Paired primer extensions with 32P linked dCTP or TTP will reveal whether a Cytosine is methylated or not. Primers are made that anneal to the PCR template and terminate subsequently 5 prime of the cytosine to be assayed. This will quantitatively assess the ratio of methylated and unmethylated cytosines in the DNA sample at that specific CpG dinucleotide.
Gonzalgo ML et al. Rapid quantitation of methylation differences at specific sites using methylation-sensitive single nucleotide primer extension (Ms-SNuPE). Nucleic Acids Res (1997) 25: 2529-2531