Methylation Sensitive Single Nucleotide Primer Extension

Methylation Sensitive Single Nucleotide Primer Extension Ms-SNuPE

This method allows you to analyze several CpG dinucleotides in a single reaction. Paired primer extensions with 32P linked dCTP or TTP will reveal whether a Cytosine is methylated or not. Primers are made that anneal to the PCR template and terminate subsequently 5 prime of the cytosine to be assayed. This will quantitatively assess the ratio of methylated and unmethylated cytosines in the DNA sample at that specific CpG dinucleotide.

References:

Gonzalgo ML et al. Rapid quantitation of methylation differences at specific sites using methylation-sensitive single nucleotide primer extension (Ms-SNuPE). Nucleic Acids Res (1997) 25: 2529-2531

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