Top Hits

OligoAnalyzer 3.0 - http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx/oligocalc.asp OligoAnalyzer 3.0 allows you to analyze hairpin, self-dimer, heter-dimer, and other basic parameters of your oligo sequences. - [Read more]

Chromosome Conformation Capture (3C) - http://www.epigenome-noe.net/researchtools/protocol.php?protid=6 Chromosome Conformation Capture (3C) Protocol developed by Alice Horton & Peter Fraser - Laboratory of Chromatin and Gene Expression, Cambridge - [Read more]

Epigenetic Conference List - http://www.epigenome-noe.net/newsevents/events.php?catid=1 A list of all the conferences and meetings concerning epigenetics, from The Epigenome Site, which is excellent. - [Read more]

Advantages and Disadvantages of Monoclonal Antibodies - http://pathimmuno.wustl.edu/facilities/facilitieshybridaddisad.html Advantages and Disadvantages of Monoclonal Antibodies - [Read more]

Bisulfite Treatment of DNA - http://www.mdanderson.org/departments/methylation/display.cfm?id=955D5740-37D4-444F-ACF0193F8EC3989B&method=displayFull Bisulfite Treatment of DNA Protocol from the University of Texas, MD Anderson Cancer Center. - [Read more]

MethPrimer - Design Primers for Methylation PCRs - http://www.urogene.org/methprimer/ MethPrimer is a program for designing bisulfite-conversion-based Methylation PCR Primer. Currently, it can design primers for two types of methylation mapping PCRs: 1) Methylation-Specific PCR (MSP) and 2) Bisulfite-Sequencing PCR (BSP) or Bisulfite-Restriction PCR. - [Read more]

Combined Bisulfite Restriction Analysis of DNA (COBRA) - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1087791 Combined Bisulfite Restriction Analysis of DNA COBRA - [Read more]

Rubidium Chloride method for Transformation Competent E. coli - http://www.nwfsc.noaa.gov/announcements/micro-server.html Rubidium Chloride method for Transformation Competent E. coli - [Read more]

Molarity Calculator - http://www.graphpad.com/quickcalcs/Molarityform.cfm Molarity Calculator - [Read more]

Chromas Lite - http://www.technelysium.com.au/chromas_lite.html Opens chromatogram files from Applied Biosystems and Amersham MegaBace DNA sequencers. Opens SCF format chromatogram files created by ALF, Li-Cor, Visible Genetics OpenGene, Beckman CEQ 2000XL and CEQ 8000, and other sequencers. Save in SCF or Applied Biosystems format. Prints chromatogram with options to zoom or fit to one page. Exports sequences in plaint text or FASTA format. ++ Much More !! - [Read more]

ChIP Chromatin Immunoprecipitation Assay Tips - http://www.abcam.com/index.html?rid=310 Supplement your protocols - useful tips for chromatin IP compiled by Dr. Andy Bannister and the Abcam Chromatin Team. - [Read more]

Bisulphite Genomic Sequencing Protocol - http://www.methdb.net/ Bisulphite Genomic Sequencing Protocol, Methods and Materials. - [Read more]

Chromatin Immunoprecipitation (ChIP) Assay - http://www.epigenome-noe.net/researchtools/protocol.php?protid=10 Chromatin Immunoprecipitation (ChIP) assays are used to evaluate the association of proteins with specific DNA regions. Developed by Antigone Kouskouti & Irene Kyrmizi Institute of Molecular Biology and Biotechnology FORTH 1527 Vassilika Vouton 711 10, Heraklion, Crete Greece - [Read more]

TSA Treatment of Mammalian Cells - http://www.epigenome-noe.net/researchtools/protocol.php?protid=11 TSA Treatment of Mammalian Cells developed by Daniéle Roche from the Almouzni Lab, Paris - [Read more]

How to decontaminate tissue culture samples - by Invitrogen - http://invitrogen.custhelp.com/cgi-bin/invitrogen.cfg/php/enduser/std_adp.php?p_sid=HczfJTng&p_lva=1&p_faqid=69 How to decontaminate tissue culture samples - by Invitrogen - a great protocol and procedure to follow for decontamination. - [Read more]

DNA Methylation Database - http://www.methdb.net/ This database has been established to provide the scientific and research community with a large resource that is available to store DNA methylation data and to make such data readily available to the public for study and access. - [Read more]

Methylation PCR - http://www.mdanderson.org/departments/methylation/display.cfm?id=0CB11381-59AE-4D90-AC0B343F2D646EDD&method=displayFull Methylation-Specific PCR. Protocol by James Herman. Basic Protocol, Critical parameters. The University of Texas M. D. Anderson Cancer Center - [Read more]

EMBOSS CpGPlot CpGReport - http://www.ebi.ac.uk/emboss/cpgplot/ CpG Plot is a program that plots CpG rich areas, and CpG Report reports all CpG rich regions. - [Read more]

Decontamination of cells from the yeast - http://www.geocities.com/mesentsev/Biblioteque/decontamination.html Decontamination of cells from the yeast - a great protocol for use when you are in trouble from yeast. - [Read more]

Dragon Promoter Finder - http://research.i2r.a-star.edu.sg/promoter/promoter1_5/DPF.htm Program finds promoters and promoter relevant sequences within any set of sequence. - [Read more]