Epigenetic DNA Methylation Station

Expression Protocol in M9 Minimal Media via T7 Promoter - Details

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ID:515
Title: Expression Protocol in M9 Minimal Media via T7 Promoter - http://structbio.vanderbilt.edu/chazin/wisdom/labpro/M9.html
Description:Expression Protocol in M9 Minimal Media via T7 Promoter - The following protocol has been used successfully to 15N or 13C/15N label our proteins using our pET1120/BL21(DE3) expression system: Preparing M9 minimal media begins with preparing a 5x stock solution of M9 salts.
Category:Media, Solutions and Dilutions: Culture Media & Plates
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Date Added:May 31, 2007 09:03:30 PM
Number Hits:8
 
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