Epigenetic DNA Methylation Station

Expression Protocol in M9 Minimal Media via T7 Promoter - Link

Information 

Title:

Expression Protocol in M9 Minimal Media via T7 Promoter

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http://structbio.vanderbilt.edu/chazin/wisdom/labpro/M9.html

Description:

Expression Protocol in M9 Minimal Media via T7 Promoter - The following protocol has been used successfully to 15N or 13C/15N label our proteins using our pET1120/BL21(DE3) expression system: Preparing M9 minimal media begins with preparing a 5x stock solution of M9 salts.

Category:

Media, Solutions and Dilutions: Culture Media & Plates

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Epigenetics