Assays, protocols and procedures to identify colonies, plasmid assays, bacteria colony PCR, screening for recombinant colonies, and transfer of DNA to nylon membranes or nitrocellulose.
| Bacterial Colony PCR - http://www.cbs.umn.edu/~kclark/protocols/bactPCR.html This protocol allows rapid detection of transformation success when primers are available to allow determination of correct ligation products by size or hybridization. - [Read about Bacterial Colony PCR] |
| Method: Transfer of DNA from Bacterial Colonies to Nylon Membrane or Nitrocellulose - http://humgen.wustl.edu/hdk_lab_manual/plasmid/plsmid04.html Method: Transfer of DNA from Bacterial Colonies to Nylon Membrane or Nitrocellulose - To transfer DNA from bacterial colonies to nylon (or nitrocellulose) membrane so that a library can be screened by hybridization. - [Read about Method: Transfer of DNA from Bacterial Colonies to Nylon Membrane or Nitrocellulose] |
| Screening of recombinants - http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/colony.html Screening of recombinants - [Read about Screening of recombinants] |
| Method: Toothpick Plasmid Assay - http://humgen.wustl.edu/hdk_lab_manual/plasmid/plsmid03.html The toothpick assay is used to identify bacterial colonies containing recombinant plasmids following transformation and to obtain an approximation of the plasmid insert sizes. - [Read about Method: Toothpick Plasmid Assay] |
| Maintenance of Probes in bacteria including Escherichia coli - http://wheat.pw.usda.gov/~lazo/methods/lazo/bactstor.html Maintenance of Probes in bacteria including Escherichia coli - [Read about Maintenance of Probes in bacteria including Escherichia coli] |
| LB and LB+ Amp Plates Preparation - http://www.whitelabs.org/1.0/Lab%20Protocols/bacterial%20protocols/Preparation%20of%20LB%20and%20LB.htm LB and LB+ Amp Plates Preparation from White Lab. - [Read about LB and LB+ Amp Plates Preparation] |