Electroporation links, protocols and recipes, with detailed descriptions and methods and materials.
| ELECTROPORATION OF EUKARYOTIC CELLS - http://omrf.ouhsc.edu/~frank/EPOREUK.html This protocol uses a Bio-Rad Gene Pulser II apparatus to electroporate DNA into human cell lines. The cells should first be centrifuged, then resuspended in fresh medium without fetal calf serum for electroporation. - [Read more] |
| Method for Single-Cell Electroporation - http://www.cshl.edu/labs/cline/sce.html Method and Protocol for Single-Cell Electroporation. Single-cell electroporation (SCE) is a technique we have developed to deliver genes into individual cells within intact tissues, although it may also be applicable to cells in disperse cultures. - [Read more] |