Information and Protocols Links to all the information available on the internet for cloning, including BAC cloning, colony screening, construction of genome DNA libraries, ET cloning, library screening, PCR product cloning, vector preparation and TA and YAC cloning. Preparation of DNA Fragments for Insertion into Vector Preparation of Cleaved Plasmid DNA Ligation Preparation of Competent Cells Transformation
BAC Library (1)BAC Cloning (18)cDNA Cloning (3)cDNA Library Protocols (21)Cloning Strategies (7)Cloning Tools (4)Cloning Vectors (10)Colony Screening (8)Cosmid Cloning (6)DNA Cloning (14)DNA Library Protocol (4)ET Cloning (0)Genome DNA Library Protocols (5)Human Cloning (4)Lentiviral Cloning (0) | Lentiviral Vectors (0)Library Screening (0)Ligation Reaction (0)Oligo Cloning (0)PCR Product Cloning (0)Phage Library Cloning (1)RNA Cloning (1)Shotgun Cloning (0)Stem Cell Cloning (1)Subcloning (1)Subtractive Cloning (0)TA Cloning (0)Topo TA Cloning (0)Vector Preparation (2)YAC Cloning (0) |
| Subcloning Procedures - http://www.its.caltech.edu/%7Ebjorker/Martin_subcloning%20procd.pdf Extensive 7 page PDF protocol on all cloning procedures and techniques. Great link ! - [Read about Subcloning Procedures] |
| Subcloning Tips from the Experts - http://www.its.caltech.edu/%7Ebjorker/PJB%27s_cloning_hints.pdf Expert tips for cloning. - [Read about Subcloning Tips from the Experts] |
| Vector systems for cloning different sizes of DNA fragments - http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=hmg.section.406 Cell-based DNA cloning has been used widely as a tool for producing quantities of pure DNA for physical characterization and functional studies of individual genes, gene clusters or other DNA sequences of interest. However, the size of different DNA sequences of interest can vary enormously (e.g. human gene sizes are known to vary between 0.1 kb and 2.5 Mb). - [Read about Vector systems for cloning different sizes of DNA fragments] |
| Subtractive Cloning - http://web.wi.mit.edu/sive/pub/Lab%20Resources%20Documents/Sagerstrom%20SubCloning.pdf Subtractive cloning is a powerful technique that allows isolation of the differences in the nucleic acid composition of two cell samples. This is a paper on subtractive cloning from Annu. Rev. Biochem. 1997. - [Read about Subtractive Cloning] |
| Quick Shotgun Cloning of Phage Inserts - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/molecularCloning/phageInsertSubcloning.html Quick Shotgun Cloning of Phage Inserts Protocol and Information Links from the University of Zurich. - [Read about Quick Shotgun Cloning of Phage Inserts] |
| Random subclone generation - http://www.genome.ou.edu/protocol_book/protocol_partII.html#II Random subclone generation protocol with information and directions on sonication, nebulization, Random fragment end-repair, size selection, and phosphorylation, DNA ligation, Competent cell preparation, Calcium Chloride treated bacterial cell transformation, and Microcentrifuge Tube Transformation. - [Read about Random subclone generation] |
| Acetate Precipitation DNA Isolation - http://www.genome.ou.edu/DblAcetateProcV3.html Acetate Precipitation DNA Isolation Protocol through a Clear Lysate. - [Read about Acetate Precipitation DNA Isolation] |
| Molecular Cloning - http://www.fpl.fs.fed.us/documnts/pdf1989/schal89a.pdf Molecular Cloning, Basic Method Article. - [Read about Molecular Cloning] |
| Physicla Mapping Cloning - http://www.apsnet.org/mpmi/PDFS/1997/1014-01N.pdf Physicla Mapping through Cloning. - [Read about Physicla Mapping Cloning] |
| Cloning Tutorial - http://www.macvector.com/Resources/ClickCloningTutorial.pdf Cloning Tutorial, great for learning cloning and the cloning procedure. - [Read about Cloning Tutorial] |
| Cloning Review - http://opa.faseb.org/pdf/cloning.pdf Cloning Review Paper. Great. Discusses the past, present and the future on cloning. - [Read about Cloning Review] |
| DNA Cloning Course - http://www.sabc.murdoch.edu.au/courses/DNABrochure_web.pdf DNA Cloning Course from murdochuniversity. - [Read about DNA Cloning Course] |
| Cloning Vector Basics - http://jbpc.mbl.edu/GenomesCourse/media/200510171900-vectors.pdf Cloning Vector Basics, for genomic, cDNA and subcloning. - [Read about Cloning Vector Basics] |
| Recombinant DNA and Cloning - http://biochem.med.ufl.edu/images/MolBiol04/Lecture%2054-2.pdf Recombinant DNA and Cloning lecture. - [Read about Recombinant DNA and Cloning] |