Preparing culture media and plates, stock solutions and ampicillin agar plates, selection of clones, plate supplements and broth and agar plates.
| M9 Plate Supplementation Table - http://www.fhcrc.org/science/labs/gottschling/bac/bsup.html M9 Plate Supplementation Table - [Read about M9 Plate Supplementation Table] |
| Preparation of Broth and Plates - http://people.virginia.edu/~gwl6s/protocols/recipes.html Preparation of Broth and Plates - LB Broth , NZY , Terrific Broth Combine, LB Plates Broth Combine, NZY Plates Combine, NZY Top Agarose , Antibiotics , SM Buffer Combine, MgSO4 and Maltose. - [Read about Preparation of Broth and Plates] |
| Expression Protocol in M9 Minimal Media via T7 Promoter - http://structbio.vanderbilt.edu/chazin/wisdom/labpro/M9.html Expression Protocol in M9 Minimal Media via T7 Promoter - The following protocol has been used successfully to 15N or 13C/15N label our proteins using our pET1120/BL21(DE3) expression system: Preparing M9 minimal media begins with preparing a 5x stock solution of M9 salts. - [Read about Expression Protocol in M9 Minimal Media via T7 Promoter] |
| Bacterial Media Solutions and Stocks - http://humgen.wustl.edu/hdk_lab_manual/plasmid/plsmid11.html Bacterial Media Solutions and Stocks - [Read about Bacterial Media Solutions and Stocks] |
| Agar Plates for Selection of Clones in Bacteria - http://www.le.ac.uk/biology/phh4/bactplte.htm Agar Plates for Selection of Clones in Bacteria - [Read about Agar Plates for Selection of Clones in Bacteria] |
| media - http://rothlab.ucdavis.edu/protocols/M10.html media - [Read about media] |