Microarray
Protocols on microarray experiments and array fabrication, probe preparation and hybridization. Also a list of numerous protocols intended to serve as a basic introduction to making and using cDNA microarrays.
Categories:
Microarray Data Analysis (2)Microarray Hybridization (4)Nucleic Acid Labeling (3)Slide Preparation (1)Tissue Microarray Data Analysis (2) |
Links:
| A Consise Guide to cDNA Microarray Analysis - http://pga.tigr.org/PDF/BiotechniquesCookbook_II.pdf The Institute for Genomic Research, Rochville, MD, Consice Guide to cDNA Microarray Analysis developed by Hegde et al., 2000. - [Read more] |
| A Scanning Array - http://fds.oup.com/www.oup.co.uk/pdf/pas/6-5-3.pdf Protocol for making a scanning microarray, in situ synthesis of oligonucleotides on a solid substrate - [Read more] |
| Cluster Analysis and Visualization - http://rana.lbl.gov/EisenSoftware.htm Cluster Analysis and Visualization - Perform a variety of types of cluster analysis and other types of processing on large microarray datasets. Currently includes hierarchical clustering, self-organizing maps (SOMs), k-means clustering, principal component analysis.Hierarchical clustering methods described in Eisen et al. (1998) PNAS 95:14863. - [Read more] |
| Fluorescent cDNA Microarray Technology Protocol - http://research.nhgri.nih.gov/microarray/protocols.html There are three fundamental types of operations required in a cDNA microarray experiment. The first step is cDNA AMPLIFICATION AND PRINTING. The second step is RNA EXTRACTION AND LABELING. The last step is HYBRIDIZATION AND DATA EXTRACTION. This link reviews and covers all needed topics very well. - [Read more] |
| Microarray Artifact Tool - http://discover.nci.nih.gov/affytools/ This page provides tools to assess regional biases and artifacts on your microarrays. - [Read more] |
| Microarray Protocol - http://www.biotechnologycenter.org/hio/microarray/microprocedures/mapr.pdf A protocol for microarrays, including all steps from total RNA isolation to hybridization of dendrimers to the array and washing unbound dendrimer from the glass array. - [Read more] |
| Vodkin Lab Microarray Protocol - http://soybeangenomics.cropsci.uiuc.edu/protocols/index.html Complete Microarray Protocol: Preparation of DNA, Electrophoresis of PCR products, Purification of PCR products with Sephadex, Preparing poly-L-lysine coated slides, Postprint slide treatments, Postprint blocking of amine slides, Postprint blocking of aldehyde slides, Reverse transcriptase labeling with Cy3/Cy5, Staining of total DNA on slides, Microarray construction, Laser scanning Spot quantitation, Statistics of expression Sample tracking, Controls used in validating microarrays - [Read more] |
