Epigenetic Research Protocols

Chromosome Conformation Capture (3C) Protocol developed by Alice Horton & Peter Fraser - Laboratory of Chromatin and Gene Expression, Cambridge - [Read about Chromosome Conformation Capture (3C)]

TSA Treatment of Mammalian Cells developed by Daniéle Roche from the Almouzni Lab, Paris - [Read about TSA Treatment of Mammalian Cells]

RNA FISH on cultured cells in interphase developed by Karen Ng from Wutz Lab, Vienna - [Read about RNA FISH on cultured cells in interphase]

Preparation and Immunostaining of Polytene Chromosome Squashes developed by Christine James, Paul Badenhorst & Bryan Turner from the well-known Institute of Biomedical Research, Birmingham - [Read about Preparation and Immunostaining of Polytene Chromosome Squashes]

Immunofluorescence and Fluorescent In Situ Hybridization in Mouse Fibroblasts and Embryonic Stem Cells developed by a group from Paris, Julie Chaumeil & Edith Heard - Mammalian Development Epigenetics Group, Paris - [Read about Immunofluorescence and Fluorescent In Situ Hybridization in Mouse Fibroblasts and Embryonic Stem Cel]

Multicolor 3D-FISH in vertebrate cells developed by Marion Cremer, Claudia Weierich, Irina Solovei at AG Martinsried-Planegg - [Read about Multicolor 3D-FISH in vertebrate cells]

Nucleosome mobilization assay developed by Karim Bouazoune - Massachusetts General Hospital, Boston - [Read about Nucleosome mobilization assay]

Live imaging with Drosophila tissue culture cells developed by Patrick Heun and the team at MPI of Immunobiology, Freiburg. - [Read about Live imaging with Drosophila tissue culture cells]

Two-colour fluorescent in situ DNA hybridization on whole mount Drosophila embryos and larval imaginal discs created by Frédéric Bantignies, Charlotte Grimaud & Giacomo Cavalli from the Institute of Human Genetics, Montpellier - [Read about Two-colour fluorescent in situ DNA hybridization on whole mount Drosophila embryos and larval imagin]

Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides Leonie Ringrose - ZMBH, Heidelberg - [Read about Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides]

Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes developed by Charlotte Grimaud, Frédéric Bantignies & Giacomo Cavalli - Institute of Human Genetics, Montpellier - [Read about Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes]

Protocols to reveal Epigenetic Mechanisms. - [Read about Epigenetic Mechanisms]

Site-directed hydroxy radical mapping of nucleosome positions in vitro developed by the team , Andrew Flaus, Chris Stockdale, Elisa Garcia-Wilson, Maik Engeholm, Helder Ferreira and Tom Owen-Hughes from the well-known Division of Gene Regulation & Expression, Dundee - [Read about Site-directed hydroxy radical mapping of nucleosome positions in vitro]

Linear amplification of limiting amounts of RNA for gene expression studies developed by the team of Alexandra Schebesta & Shane McManus - Busslinger Lab, Vienna - [Read about Linear amplification of limiting amounts of RNA for gene expression studies]

RNase A Treatment of Mouse Cells developed by Christèle Maison and the renowned Almouzni Lab, Paris - [Read about RNase A Treatment of Mouse Cells]

Tracking individual chromosomes with integrated arrays of Lacop sites and GFP-Laci repressor developed by Frank Neumann, Angela Taddei & Susan Gasser from the world-renowned The Friedrich Miescher Institute for Biomedical Research, Basel - [Read about Tracking individual chromosomes with integrated arrays of Lacop sites and GFP-Laci repressor]