Epigenetic Research Protocols

Chromosome Conformation Capture (3C) - http://www.epigenome-noe.net/researchtools/protocol.php?protid=6 Chromosome Conformation Capture (3C) Protocol developed by Alice Horton & Peter Fraser - Laboratory of Chromatin and Gene Expression, Cambridge - [Read more]

Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides - http://www.epigenome-noe.net/researchtools/protocol.php?protid=2 Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides Leonie Ringrose - ZMBH, Heidelberg - [Read more]

Epigenetic Mechanisms - http://rosenfeldlab.ucsd.edu/NEU221/publications/mager_2005.pdf Protocols to reveal Epigenetic Mechanisms. - [Read more]

Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes - http://www.epigenome-noe.net/researchtools/protocol.php?protid=4 Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes developed by Charlotte Grimaud, Frédéric Bantignies & Giacomo Cavalli - Institute of Human Genetics, Montpellier - [Read more]

Immunofluorescence and Fluorescent In Situ Hybridization in Mouse Fibroblasts and Embryonic Stem Cel - http://www.epigenome-noe.net/researchtools/protocol.php?protid=3 Immunofluorescence and Fluorescent In Situ Hybridization in Mouse Fibroblasts and Embryonic Stem Cells developed by a group from Paris, Julie Chaumeil & Edith Heard - Mammalian Development Epigenetics Group, Paris - [Read more]

Linear amplification of limiting amounts of RNA for gene expression studies - http://www.epigenome-noe.net/researchtools/protocol.php?protid=20 Linear amplification of limiting amounts of RNA for gene expression studies developed by the team of Alexandra Schebesta & Shane McManus - Busslinger Lab, Vienna - [Read more]

Live imaging with Drosophila tissue culture cells - http://www.epigenome-noe.net/researchtools/protocol.php?protid=25 Live imaging with Drosophila tissue culture cells developed by Patrick Heun and the team at MPI of Immunobiology, Freiburg. - [Read more]

Multicolor 3D-FISH in vertebrate cells - http://www.epigenome-noe.net/researchtools/protocol.php?protid=23 Multicolor 3D-FISH in vertebrate cells developed by Marion Cremer, Claudia Weierich, Irina Solovei at AG Martinsried-Planegg - [Read more]

Nucleosome mobilization assay - http://www.epigenome-noe.net/researchtools/protocol.php?protid=24 Nucleosome mobilization assay developed by Karim Bouazoune - Massachusetts General Hospital, Boston - [Read more]

Preparation and Immunostaining of Polytene Chromosome Squashes - http://www.epigenome-noe.net/researchtools/protocol.php?protid=1 Preparation and Immunostaining of Polytene Chromosome Squashes developed by Christine James, Paul Badenhorst & Bryan Turner from the well-known Institute of Biomedical Research, Birmingham - [Read more]

RNA FISH on cultured cells in interphase - http://www.epigenome-noe.net/researchtools/protocol.php?protid=7 RNA FISH on cultured cells in interphase developed by Karen Ng from Wutz Lab, Vienna - [Read more]

RNase A Treatment of Mouse Cells - http://www.epigenome-noe.net/researchtools/protocol.php?protid=12 RNase A Treatment of Mouse Cells developed by Christèle Maison and the renowned Almouzni Lab, Paris - [Read more]

Site-directed hydroxy radical mapping of nucleosome positions in vitro - http://www.epigenome-noe.net/researchtools/protocol.php?protid=21 Site-directed hydroxy radical mapping of nucleosome positions in vitro developed by the team , Andrew Flaus, Chris Stockdale, Elisa Garcia-Wilson, Maik Engeholm, Helder Ferreira and Tom Owen-Hughes from the well-known Division of Gene Regulation & Expression, Dundee - [Read more]

Tracking individual chromosomes with integrated arrays of Lacop sites and GFP-Laci repressor - http://www.epigenome-noe.net/researchtools/protocol.php?protid=15 Tracking individual chromosomes with integrated arrays of Lacop sites and GFP-Laci repressor developed by Frank Neumann, Angela Taddei & Susan Gasser from the world-renowned The Friedrich Miescher Institute for Biomedical Research, Basel - [Read more]

TSA Treatment of Mammalian Cells - http://www.epigenome-noe.net/researchtools/protocol.php?protid=11 TSA Treatment of Mammalian Cells developed by Daniéle Roche from the Almouzni Lab, Paris - [Read more]

Two-colour fluorescent in situ DNA hybridization on whole mount Drosophila embryos and larval imagin - http://www.epigenome-noe.net/researchtools/protocol.php?protid=5 Two-colour fluorescent in situ DNA hybridization on whole mount Drosophila embryos and larval imaginal discs created by Frédéric Bantignies, Charlotte Grimaud & Giacomo Cavalli from the Institute of Human Genetics, Montpellier - [Read more]