Information and protocols for establishing and maintaining primary cultures for cell culture.
| Cell Isolation Techniques - http://www.tissuedissociation.com/techniques.html Cell Isolation Techniques - the best link for information, protocols and methods for cell isolation techniques. Highly recommended for cell isolation techniques ! - [Read more] |
| Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion - http://www.animal.ufl.edu/hansen/protocols/endocult.htm This method was originally developed in the laboratories of R.M. Roberts and F.W. Bazer to allow metabolic labelling of secretory proteins synthesized de novo by cultured porcine endometrium (J. Reprod. Fertil. 60:41-48, 1980). It has since been used to study secretion of proteins and prostaglandins by endometrium from the cow, ewe, mare, bitch and other species. The technique is also useful for culture of peri-implantation conceptuses and placental tissues for metabolic labelling studies. - [Read more] |
| Establishment of a Primary Culture - http://homepages.gac.edu/~cellab/chpts/chpt12/ex12-10.html Establishment of a Primary Culture - [Read more] |
| ISOLATION OF T CELLS FROM SYNOVIAL MEMBRANES - http://omrf.ouhsc.edu/~frank/TCELLISO.html ISOLATION OF T CELLS FROM SYNOVIAL MEMBRANES - [Read more] |
| Slice and Explant Culture Protocols - http://faculty.washington.edu/rhevner/Slice_Culture.doc For axon tracing & cell culture studies in vitro using embyros age E11.5 – E16.5 modified from Rubenstein lab and Price lab protocols. - [Read more] |