Information and protocol links to cell harvesting. Includes trypsinizing cells, cloning cylinder use for harvesting cell colonies, single cell cloning by serial dilution.
| Method: Preparation of Lymphocyte Cell Pellet for Storage - http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc5.html Method: Preparation of Lymphocyte Cell Pellet for Storage - Following propagation to 1 X 108 cells, lymphoblastoid cells are conveniently stored at -80 degrees C to preserve the high molecular weight DNA in the cells until the DNA is purified. This procedure describes the steps required to harvest and freeze the cells for long term storage. - [Read more] |
| Single Cell Cloning by Serial Dilution - http://www.corning.com/Lifesciences/technical_information/techDocs/Single_cell_cloning_protocol.pdf Single Cell Cloning by Serial Dilution - [Read more] |
| Trypsinization of Adherent Cells - http://www.musc.edu/BCMB/ceramide/protocols/0005.html Trypsinization of Adherent Cells - [Read more] |
| Trypsinizing cells - http://pingu.salk.edu/~sefton/Hyper_protocols/trypsin.html Trypsinizing cells - [Read more] |
| Use of Corning Cloning Cylinders for Harvesting Cell Colonies - http://www.corning.com/Lifesciences/technical_information/techDocs/cc_cloning_protocol_1_03_cls_an_041w.pdf Use of Corning Cloning Cylinders for Harvesting Cell Colonies pdf. - [Read more] |