Epigenetic Methylation Station

Your source for information, protocols, and bioinformatics about epigenetics and DNA methylation.

Epigenetic Methylation Station

Your source for information, protocols, and bioinformatics about epigenetics and DNA methylation.
PCR Fluorescence Melting Curve Analysis 30Sep

PCR Fluorescence Melting Curve Analysis

This method utilizes both treatment of DNA with sodium bisulfite initially, and melting curve analysis by using a thermal cycler, like the LightCycler from Roche Diagnostics, which uses a DNA-binding dye that binds double-stranded DNA,